Inoue K, Sobue H, Minagawa M, Tanimura K, Ueki K
No To Shinkei. 1978 Nov;30(11):1215-9.
A rat brain tumor model was prepared by semi-stereotactically implanting 1 x 10(6) RG--C6 tumor cells (chemically induced and maintained in vitro) in the right caudate nucleus of inbred WKA rat by a new method "Soft Agar Technique" which we developed. The tumor grew at a nearly fixed rate. We measured the maximum tumor area and the whole brain area of the rat brain which were sectioned coronary in series. We regarded the percentage of the tumor area to the whole area of the same section as tumor growth index. Microcytotoxicity assays of peripheral blood lymphocytes were performed at various stages of the tumor growth. RG--C6 cells were used as target cells, and they mixed with lymphocytes by Takasugi and Klein's method. Cytotoxicity index was higher in 22 cases (80.00 +/- 16.67; tumor growth index, 0--10) than in 28 cases of the control group (67. 25 +/- 19.68) and then gradually decreased with tumor growth (Fig. 2). At last cytotoxicity index went down to 41.55 +/- 24.94 in 11 cases (tumor growth index, over 31). These observations have a strong resemblance to other animal tumor models and suggest that the rat having brain tumor intra-axially have concomitant immunity especially in the first stage of tumor bearing.
采用我们研发的“软琼脂技术”这一新方法，将1×10(6)个RG - C6肿瘤细胞（化学诱导并在体外维持培养）半立体定向植入近交系WKA大鼠的右侧尾状核，制备大鼠脑肿瘤模型。肿瘤以近乎固定的速率生长。我们测量了大鼠脑的最大肿瘤面积和全脑面积，这些脑是连续冠状切片的。我们将同一切片中肿瘤面积占整个面积的百分比视为肿瘤生长指数。在肿瘤生长的各个阶段进行外周血淋巴细胞的微细胞毒性测定。以RG - C6细胞作为靶细胞，按照高杉和克莱因的方法将其与淋巴细胞混合。22例（80.00±16.67；肿瘤生长指数为0 - 10）的细胞毒性指数高于对照组的28例（67.25±19.68），然后随着肿瘤生长逐渐降低（图2）。最后，11例（肿瘤生长指数超过31）的细胞毒性指数降至41.55±24.94。这些观察结果与其他动物肿瘤模型有很强的相似性，表明脑内有肿瘤的大鼠具有伴随免疫，尤其是在荷瘤的第一阶段。
