Staley T E, Redmond C F, Gilliland S E, Bush L J
Am J Vet Res. 1981 Jun;42(6):912-7.
The uptake and incorporation of [14C]glucosamine into secreted intestinal glycoproteins was examined in the calf intestinal mucosa and isolated epithelial cells, using an organ culture system. Incorporation varied with the area of the intestine, ie duodenum greater than jejunum greater than ileum. Secreted glycoproteins were examined after 3 and 24 hours in organ culture. Initially, a glycoprotein approximately 68,000 molecular weight (mol wt) was secreted by all areas of the intestine. This was followed by the appearance of a larger glycoprotein 10(6) to 10(7) mol wt. Enzymatic cleavage of jejunal brush borders released a glycocalyx glycoprotein similar in size to the secreted large mol wt glycoprotein. These similarities in mol wt are only suggestive evidence that the 2 large glycoproteins are the same. This procedure allows for the study of the maturation and development of a poorly understood component of the intestinal epithelial cell which has a wide range of functional considerations.
利用器官培养系统，在小牛肠黏膜和分离的上皮细胞中检测了[14C]葡糖胺摄取并掺入分泌型肠糖蛋白的情况。掺入情况因肠段不同而有所差异，即十二指肠大于空肠大于回肠。在器官培养3小时和24小时后对分泌的糖蛋白进行了检测。最初，所有肠段均分泌一种分子量约为68,000的糖蛋白。随后出现了一种分子量为10(6)至10(7)的更大糖蛋白。空肠刷状缘的酶解作用释放出一种糖萼糖蛋白，其大小与分泌的大分子糖蛋白相似。分子量上的这些相似性只是提示性证据，表明这两种大分子糖蛋白是相同的。该方法有助于研究肠上皮细胞中一个了解甚少但具有广泛功能考量的成分的成熟和发育过程。
