Crabtree J E, Heatley R V, Losowsky M S
Department of Medicine, St James's University Hospital, Leeds.
Gut. 1989 Oct;30(10):1339-43. doi: 10.1136/gut.30.10.1339.
Glycoprotein biosynthesis by jejunal mucosa was examined during culture in vitro in 26 patients with coeliac disease and 19 controls. The incorporation rates of tritiated glucosamine into tissue and secreted glycoproteins were determined using established techniques. The total glucosamine incorporation in untreated coeliac patients was significantly greater than that of histologically normal mucosa (p less than 0.001) and jejunal tissue from patients with treated coeliac disease (p less than 0.01). Enhanced secretion of in vitro labelled glycoproteins was observed in untreated coeliac patients. The total incorporation of tritiated glucosamine in intestinal tissues was correlated with goblet cell numbers. These results indicate that quantitative changes in glycoprotein synthesis and secretion occur in coeliac disease.
在体外培养过程中,对26例乳糜泻患者和19名对照者的空肠黏膜糖蛋白生物合成进行了检测。采用既定技术测定了氚标记葡糖胺掺入组织和分泌糖蛋白的速率。未经治疗的乳糜泻患者中葡糖胺的总掺入量显著高于组织学正常的黏膜(p<0.001)以及经治疗的乳糜泻患者的空肠组织(p<0.01)。在未经治疗的乳糜泻患者中观察到体外标记糖蛋白的分泌增强。氚标记葡糖胺在肠道组织中的总掺入量与杯状细胞数量相关。这些结果表明,乳糜泻中糖蛋白合成和分泌发生了定量变化。