Brown D T, Marriott C, Beeson M F, Barrett-Bee K
Am Rev Respir Dis. 1981 Sep;124(3):285-91. doi: 10.1164/arrd.1981.124.3.285.
A rheologically active mucin fraction was isolated from pooled sputum and was shown to be free of any major protein contaminants. The procedure used allows the isolation to be carried out by a batch process so that sufficient material is available for extensive biochemical and biophysical studies. Because the separation was carried out using sputum, the purified material should prove suitable for the evaluation of drugs used in chronic obstructive airway disease. This paper describes the separation process in detail and the partial biochemical characterization of the mucin fraction.
从混合痰液中分离出一种具有流变活性的粘蛋白组分，且显示其不含任何主要蛋白质污染物。所采用的方法允许通过分批处理进行分离，从而有足够的材料用于广泛的生化和生物物理研究。由于分离是使用痰液进行的，纯化后的材料应证明适用于评估慢性阻塞性气道疾病中使用的药物。本文详细描述了分离过程以及粘蛋白组分的部分生化特性。
