Dowd P M, Kirby J D, Holborow E J, Cooke E D, Bowcock S A
Br J Dermatol. 1981 Aug;105(2):179-88. doi: 10.1111/j.1365-2133.1981.tb01203.x.
Phagocytosed immune complexes in polymorphonuclear leukocytes of nineteen patients with Raynaud's phenomenon were detected by scoring the cells for IgG and C3 inclusions. Parallel estimations of circulating immune complexes were made by liquid phase C1q binding and polyethylene glycol (PEG) precipitation methods. The results were compared with the degree of cold stress-induced vasospasm measured by quantified infra-red thermography. Thirteen of nineteen patients with Raynaud's phenomenon compared with only one of seventeen normal controls showed significantly elevated levels of phagocytosed IgG. The correlation of IgG and C3 scores for individual patients was high and the IgG and C3 scores correlated well with the thermographic assessment of the severity of the Raynaud's phenomenon. Two of the patients had elevated levels of circulating immune complexes detectable by raised levels of C1q binding, four patients had raised IgG and three patients raised IgM complexes detectable by PEG precipitation. None of the controls had elevated levels of circulating immune complexes detectable by either method. There was no correlation between the levels of circulating and phagocytosed immune complexes.
通过对19例雷诺现象患者多形核白细胞中的免疫复合物进行IgG和C3包涵体评分，检测吞噬的免疫复合物。采用液相C1q结合法和聚乙二醇（PEG）沉淀法对循环免疫复合物进行平行评估。将结果与通过定量红外热成像测量的冷应激诱导的血管痉挛程度进行比较。19例雷诺现象患者中有13例与17例正常对照中的仅1例相比，吞噬的IgG水平显著升高。个体患者的IgG和C3评分相关性高，且IgG和C3评分与雷诺现象严重程度的热成像评估相关性良好。2例患者通过C1q结合水平升高可检测到循环免疫复合物水平升高，4例患者IgG升高，3例患者通过PEG沉淀可检测到IgM复合物升高。两种方法均未在任何对照中检测到循环免疫复合物水平升高。循环免疫复合物水平与吞噬的免疫复合物水平之间无相关性。
