D'Anna J A, Gurley L R, Becker R R
Biochemistry. 1981 Jul 21;20(15):4501-5. doi: 10.1021/bi00518a041.
Two histone H1 fractions [H1(I) and H1(II)] and two histone H1(o) fractions (H1(o)a and H1(o)b) have been isolated from butyrate-treated Chinese hamster (line CHO) cells by guanidine hydrochloride gradient chromatography on Bio-Rex 70 ion-exchange resin. The fractions have been identified by electrophoresis and amino acid analyses. Electrophoretic analysis of cyanogen bromide treated H1(o) in long acid-urea-polyacrylamide gels suggests that H1(o)a and H1(o)b differ, at least, within the 20-30 residue fragment(s) removed by the cyanogen bromide cleavage. Shallow-gradient Bio-Rex 70 chromatography indicates that histones H1(o)a and H1(o)b are the same as the respective CHO histones, H1(III) and H1(IV), originally resolved by Gurley and co-workers [Gurley, L. R., Walters, R. A., & Tobey, R. A. (1975) J. Biol. Chem. 250, 3936]. This identification and the phosphate incorporation data of Gurley et al. (1975) reveal new features about H1(o) phosphorylation: (1) following release from G1 arrest, H1(o)a and H1(o)b become phosphorylated in late G1 prior to DNA synthesis; (2) H1(o)a and H1(o)b are phosphorylated at similar rates throughout the cell cycle. These and other data demonstrate that histone H1(o) is phosphorylated in a cell cycle dependent fashion which mimics that of histone H1.
通过在Bio-Rex 70离子交换树脂上进行盐酸胍梯度色谱，从丁酸盐处理的中国仓鼠（CHO细胞系）细胞中分离出了两种组蛋白H1组分[H1(I)和H1(II)]以及两种组蛋白H1(o)组分（H1(o)a和H1(o)b）。这些组分已通过电泳和氨基酸分析进行了鉴定。在长的酸性尿素聚丙烯酰胺凝胶中对溴化氰处理的H1(o)进行电泳分析表明，H1(o)a和H1(o)b至少在溴化氰裂解去除的20 - 30个残基片段内有所不同。浅梯度Bio-Rex 70色谱表明，组蛋白H1(o)a和H1(o)b与最初由Gurley及其同事[Gurley, L. R., Walters, R. A., & Tobey, R. A. (1975) J. Biol. Chem. 250, 3936]分离的相应CHO组蛋白H1(III)和H1(IV)相同。这一鉴定以及Gurley等人（1975年）的磷酸掺入数据揭示了关于H1(o)磷酸化的新特征：(1)从G1期阻滞释放后，H1(o)a和H1(o)b在DNA合成之前的G1晚期开始磷酸化；(2)在整个细胞周期中，H1(o)a和H1(o)b以相似的速率磷酸化。这些以及其他数据表明，组蛋白H1(o)以一种依赖细胞周期的方式磷酸化，这与组蛋白H1的磷酸化方式相似。
