Vedaldi D, Rodighiero P, Guiotto A, Bordin F, Caffieri S, Dall'Acqua F
Chem Biol Interact. 1981 Sep;36(3):275-86. doi: 10.1016/0009-2797(81)90071-5.
The study of the interactions in the ground state between 4,5'-dimethylangelicin, an angular furocoumarin, and various synthetic and natural DNA samples have evidenced the presence in the macromolecule of preferred sequences suitable for binding the small ligand. They are represented by an alternate sequence of purine and pyrimidine bases in each strand of the macromolecule, without difference between the two base pairs A-T and C-G. The study of the photochemical interactions between the same DNA samples and the 4,5'-dimethylangelicin shows that preferred sites are present in the macromolecule for the covalent addition of the furocoumarin to the macromolecule too. These sites however have more strict requirements than those useful for dark binding; they are in fact represented by alternate sequences of A-T in each strand such as those present in poly [d(A-T)] . poly-[d(A-T)]. Moreover fluorescence studies made on the same DNA samples irradiated in the presence of the furocoumarin suggest that the alternate C-G regions favour formation of 4'-5'-fluorescent adducts.
对角型呋喃香豆素4,5'-二甲基白芷素与各种合成及天然DNA样品基态相互作用的研究表明，在大分子中存在适合结合小配体的优选序列。它们由大分子每条链中嘌呤和嘧啶碱基的交替序列表示，A-T和C-G这两个碱基对之间没有差异。对相同DNA样品与4,5'-二甲基白芷素之间光化学相互作用的研究表明，大分子中也存在呋喃香豆素与大分子共价加成的优选位点。然而，这些位点比用于暗结合的位点有更严格的要求；实际上，它们由每条链中A-T的交替序列表示，例如聚[d(A-T)]·聚-[d(A-T)]中存在的序列。此外，对在呋喃香豆素存在下辐照的相同DNA样品进行的荧光研究表明，交替的C-G区域有利于形成4'-5'-荧光加合物。
