通过竞争性酶联免疫吸附测定法测量猪粪便中IgA、IgG和IgM的水平。
Measurement of porcine faecal IgA, IgG and IgM levels by a competitive enzyme-linked immunosorbent assay.
作者信息
Franz J, Corthier G
出版信息
Clin Exp Immunol. 1981 Mar;43(3):645-9.
Abstract
The concentrations of IgG, IgA and IgM were compared in faecal material of healthy and diarrhoeic pigs using a competitive enzyme-linked immunosorbent assay (ELISA). The relative concentrations of faecal immunoglobulins (Ig) in healthy piglets and adults were significantly different (P less than 0.01). IgA was the most important Ig in adult faeces while IgM was predominant in piglet faeces. IgG appears to be a relatively rare component. No significant differences could be detected between groups of healthy and diarrhoeic piglets. In adult faeces, significant changes (P less than 0.02 to P less than 0.001) in Ig relative concentrations were observed between diarrhoeic and healthy animals.
摘要
采用竞争性酶联免疫吸附测定（ELISA）法，比较健康猪和腹泻猪粪便中IgG、IgA和IgM的浓度。健康仔猪和成年猪粪便中免疫球蛋白（Ig）的相对浓度存在显著差异（P＜0.01）。IgA是成年猪粪便中最重要的Ig，而IgM在仔猪粪便中占主导地位。IgG似乎是相对稀少的成分。在健康仔猪和腹泻仔猪组之间未检测到显著差异。在成年猪粪便中，腹泻动物和健康动物之间的Ig相对浓度发生了显著变化（P＜0.02至P＜0.001）。
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