采用酶联免疫吸附测定法检测阿米巴病患者血清中的抗阿米巴IgG、IgM、IgA及IgG亚类。

Determination of antiamoebic IgG, IgM, IgA and IgG subclasses in sera from patients with amoebiasis by enzyme-linked immunosorbent assay.

作者信息

Hock G M, Foon K L, Chuen H L, Choo N G, Singh M

机构信息

Department of Microbiology, Faculty of Medicine, National University of Singapore.

出版信息

Southeast Asian J Trop Med Public Health. 1989 Sep;20(3):407-14.

PMID:2633348

Abstract

The HK-9 strain of Entamoeba histolytica was cultured axenically. A crude extract of the trophozoites was used as antigen in the enzyme-linked immunosorbent assay (ELISA) for the determination of antiamoebic IgG, IgM, IgA and IgG subclasses in sera of patients with E. histolytica infection. Sera from amoebiasis patients had significantly higher mean ELISA values for all classes and subclasses of immunoglobulins examined compared with healthy controls. The ELISA for IgG, IgM and IgA was positive for 76 (97.4%), 34 (43.6%) and 62 (79.5%) respectively of 78 amoebiasis sera. Analysis of IgG subclasses by ELISA showed that 76.7%, 25%, 33.3% and 66.6% of the patients were positive for antiamoebic IgG1, IgG2, IgG3 and IgG4 respectively. IgG1 and IgG4 were the dominant IgG subclasses involved in amoebiasis. The role of antiamoebic antibodies in amoebiasis is discussed.

摘要

溶组织内阿米巴HK - 9株进行了单栖培养。滋养体的粗提物用作酶联免疫吸附测定（ELISA）中的抗原，以测定溶组织内阿米巴感染患者血清中的抗阿米巴IgG、IgM、IgA和IgG亚类。与健康对照相比，阿米巴病患者血清中所有检测的免疫球蛋白类别和亚类的平均ELISA值均显著更高。78份阿米巴病血清中，IgG、IgM和IgA的ELISA检测阳性率分别为76份（97.4%）、34份（43.6%）和62份（79.5%）。通过ELISA分析IgG亚类显示，分别有76.7%、25%、33.3%和66.6%的患者抗阿米巴IgG1、IgG2、IgG3和IgG4呈阳性。IgG1和IgG4是参与阿米巴病的主要IgG亚类。本文讨论了抗阿米巴抗体在阿米巴病中的作用。