Baeckeland E, Heinen E
Bull Assoc Anat (Nancy). 1978 Mar;62(176):65-76.
When slices of rat palatal shelves removed by dissection from 16 days 8 hours old fetuses, just before fusion, are treated by the periodic acid Schiff reaction (PAS), colored substances may be detected especially in the epithelium of the potential fusion zone; these substances are normally observed at the free surface of the epithelium. Our histochemical research, realized with the aid of the photonic microscope and of the transmission electron microscope, shows that most of the PAS positive material consists in glycogen; at the surface, this polysaccharide remains in the cytoplasm of flat degenerating cells and cannot be confused with the "cell coat" which presents no particularity, at this level, after demonstration by ruthenium red and by lanthanum nitrate.
从16天8小时大的胎儿(即将融合前)解剖取出的大鼠腭板切片,经高碘酸希夫反应(PAS)处理后,可检测到有色物质,尤其是在潜在融合区的上皮中;这些物质通常在上皮的游离表面观察到。我们借助光子显微镜和透射电子显微镜进行的组织化学研究表明,大多数PAS阳性物质为糖原;在表面,这种多糖保留在扁平退化细胞的细胞质中,在经钌红和硝酸镧显示后,在此水平上与无特殊特征的“细胞被膜”不会混淆。