Sordillo P P, Hansen H, Jhanwar S C, Beck J, Lieberman P, Helson L
Exp Cell Biol. 1981;49(5):256-66. doi: 10.1159/000163830.
Despite the difficulty in establishing human hematopoietic tumors in nude mice, four human lymphomas were successfully heterotransplanted and passaged serially in our laboratory. Additional immunosuppression with chemotherapy, whole-body radiation or splenectomy was not required for establishment of these tumors. All four of these tumors were of the non-Hodgkin's lymphoma type. In each case the tumors in the nude mice were histologically identical to the biopsy specimens from the patient in whom they were derived. Attempts to transplant tumor from 17 patients with Hodgkin's disease or 4 patients with immunoblastic lymphadenopathy were unsuccessful. Tumors from 2 patients with chronic myelogenous leukemia and 1 with hairy cell leukemia could be grown in nude mice conditioned with whole-body radiation or cytosine arabinoside, but these tumors could not be passaged to other nude mice. Cell surface markers were determined on the four serially passaged lymphomas. These surface markers were similar to the markers on the original tumors, even after long periods of mouse-to-mouse passage. In 1 patient with fevers, night sweats and mediastinal mass in whom a diagnosis had not been made after several biopsies, examination of tumor tissue that had been transplanted from the patient to the nude mouse clearly established the diagnosis of lymphoma.
尽管在裸鼠中建立人类造血肿瘤存在困难，但在我们实验室中，4例人类淋巴瘤成功实现了异种移植并连续传代。建立这些肿瘤无需通过化疗、全身照射或脾切除术进行额外的免疫抑制。所有这4例肿瘤均为非霍奇金淋巴瘤类型。在每种情况下，裸鼠体内的肿瘤在组织学上与源自患者的活检标本相同。将17例霍奇金病患者或4例免疫母细胞性淋巴结病患者的肿瘤进行移植的尝试均未成功。2例慢性粒细胞白血病患者和1例毛细胞白血病患者的肿瘤可在经全身照射或阿糖胞苷预处理的裸鼠中生长，但这些肿瘤无法传给其他裸鼠。对4例连续传代的淋巴瘤进行了细胞表面标志物检测。即使经过长时间的鼠间传代，这些表面标志物仍与原始肿瘤上的标志物相似。1例有发热、盗汗和纵隔肿块的患者，经多次活检仍未确诊，对从该患者移植到裸鼠的肿瘤组织进行检查后，明确诊断为淋巴瘤。
