Hayashi H, Nozawa T, Hatano M, Morita S
J Biochem. 1981 Jun;89(6):1853-61. doi: 10.1093/oxfordjournals.jbchem.a133387.
Bacteriochlorophyll (Bchl) protein complexes containing light harvesting (LH) Bchls were isolated from Chromatium vinosum, and their CD spectra were measured in the near-infrared region. These isolated Bchl protein complexes retained the CD signals of LH Bchls that were observed in situ in chromatophores. The CD spectrum of fraction A (containing B890) consisted of paired positive and negative bands (a double CD) having a zero-crossing at 800 nm and a single negative band at around 900 nm attributed to the B890. For low 850 fraction B (B800 and B850), a double CD having a zero-crossing at 795 nm and a single negative band at around 850 nm attributed to the B850 were found. High 850 fraction B exhibited a double CD having a zero-crossing at 795 nm, a negative band at around 860 nm and a positive band at around 840 nm. For fraction C (B800 and B820), a double CD having a zero-crossing at 795 nm and a single negative band at around 830 nm, which was attributed to the B820, were found. The double CD was attributed to the B800 in fractions A, B, and C. There was no additional CD besides the CD of the isolated Bchl protein complexes in the CD spectra of chromatophores.
从深红红螺菌中分离出含有光捕获（LH）细菌叶绿素（Bchl）的蛋白质复合物，并在近红外区域测量了它们的圆二色光谱。这些分离出的Bchl蛋白质复合物保留了在载色体中原位观察到的LH Bchl的圆二色信号。组分A（含有B890）的圆二色光谱由一对正负带（双圆二色）组成，在800nm处有一个零交叉点，在900nm左右有一个归因于B890的单负带。对于低850组分B（B800和B850），发现了一个在795nm处有零交叉点、在850nm左右有一个归因于B850的单负带的双圆二色。高850组分B表现出一个在795nm处有零交叉点、在860nm左右有一个负带和在840nm左右有一个正带的双圆二色。对于组分C（B800和B820），发现了一个在795nm处有零交叉点、在830nm左右有一个归因于B820的单负带的双圆二色。双圆二色归因于组分A、B和C中的B800。在载色体的圆二色光谱中，除了分离出的Bchl蛋白质复合物的圆二色外，没有其他额外的圆二色。
