Hayashi H, Nozawa T, Hatano M, Morita S
J Biochem. 1982 Mar;91(3):1029-38. doi: 10.1093/oxfordjournals.jbchem.a133752.
Bacteriochlorophyll (Bchl)-protein complexes containing light-harvesting Bchls were isolated from Rhodopseudomonas palustris, and the CD spectra of these complexes were measured in the near-infrared region. These isolated Bchl-protein complexes retained the CD signals of light-harvesting Bchls that were observed in intracytoplasmic membrane preparation. Comparison of the CD spectrum of B870-reaction center complexes with that of the isolated reaction centers revealed that the peak at 860 nm and the trough at 890 nm were attributable to the B870 spectral form, and that the peak at 790 nm and the trough at 810 nm were not all attributable to the reaction center. The CD spectra of spectrally different types of B800--850 complex revealed that the magnitudes of the peak at 840--850 nm and the trough at 860--870 nm were correlated with the magnitude of the absorption peak at around 850 nm. Therefore, these positive and negative CD bands were attributable to the B850 spectral form. In a similar manner, the peak at 810--820 nm and trough at 790 nm were attributable to the B800 spectral form.
从沼泽红假单胞菌中分离出含有捕光细菌叶绿素（Bchl）的细菌叶绿素-蛋白质复合物，并在近红外区域测量了这些复合物的圆二色光谱。这些分离出的Bchl-蛋白质复合物保留了在胞内膜制备物中观察到的捕光Bchl的圆二色信号。将B870-反应中心复合物的圆二色光谱与分离出的反应中心的圆二色光谱进行比较，结果表明，860nm处的峰和890nm处的谷归因于B870光谱形式，而790nm处的峰和810nm处的谷并非都归因于反应中心。不同光谱类型的B800-850复合物的圆二色光谱表明，840-850nm处的峰和860-870nm处的谷的大小与850nm左右的吸收峰大小相关。因此，这些正负圆二色带归因于B850光谱形式。同样，810-820nm处的峰和790nm处的谷归因于B800光谱形式。
