Tolstoshev P, Haber R, Trapnell B C, Crystal R G
J Biol Chem. 1981 Sep 25;256(18):9672-9.
The rates of type I collagen synthesis in sheep lung, tendon, and skin were evaluated during the latter half of fetal development and compared with the levels of type I procollagen mRNA, quantified by molecular hybridization with a type I procollagen specific complementary DNA, and with the activity of total procollagen mRNA measured by in vitro cell-free translation. In the lung and tendon, the levels of type I procollagen mRNA and activity of total procollagen mRNA parallel collagen synthesis during development. In the skin, however, type I collagen synthesis declines sharply during fetal development, but both type I procollagen mRNA levels and total procollagen mRNA activity remain at the high levels of early development. These observations suggest that in developing lung and tendon, type I procollagen mRNA levels are likely the major determinants of the levels of type I collagen synthesis. In contrast, the dichotomy between type I procollagen mRNA levels and rates of type I collagen synthesis in the developing sheep skin suggest the skin utilizes mechanisms in addition to mRNA levels to modulate expression of the type I collagen gene.
在胎儿发育后期，对绵羊肺、肌腱和皮肤中I型胶原蛋白的合成速率进行了评估，并与通过与I型前胶原特异性互补DNA进行分子杂交定量的I型前胶原mRNA水平以及通过体外无细胞翻译测量的总前胶原mRNA活性进行了比较。在肺和肌腱中，I型前胶原mRNA水平和总前胶原mRNA活性在发育过程中与胶原蛋白合成平行。然而，在皮肤中，I型胶原蛋白合成在胎儿发育期间急剧下降，但I型前胶原mRNA水平和总前胶原mRNA活性均保持在发育早期的高水平。这些观察结果表明，在发育中的肺和肌腱中，I型前胶原mRNA水平可能是I型胶原蛋白合成水平的主要决定因素。相比之下，发育中的绵羊皮肤中I型前胶原mRNA水平与I型胶原蛋白合成速率之间的二分法表明，皮肤除了利用mRNA水平外，还利用其他机制来调节I型胶原蛋白基因的表达。
