一种用于检测抗乙酰胆碱受体抗体的快速免疫吸附放射免疫测定法。

A rapid immunoadsorbent radioimmunoassay for anti-acetylcholine receptor antibody.

作者信息

Tindall R S, Kent M, Wells L

出版信息

J Immunol Methods. 1981;45(1):1-14. doi: 10.1016/0022-1759(81)90089-2.

Abstract

Antibody to the human nicotinic acetylcholine receptor has been demonstrated in the disorder myasthenia gravis and is the pathologic factor producing the characteristic symptoms of the disorder. A rapid, quantitative and sensitive radioimmunoassay using human acetylcholine receptor, affinity labeled with iodinated alpha-bungarotoxin, and utilizing the immunoadsorbent protein A-bearing Staphylococcal aureus is described. The assay has proven as effective and more efficient than double antibody immunoprecipitation assays in diagnostic screening, evaluation of various forms of therapy, determining kinetics of receptor and antibody interaction, and the production of antireceptor antibody by peripheral blood lymphocytes in culture.

摘要

在重症肌无力疾病中已证实存在抗人烟碱型乙酰胆碱受体的抗体，且该抗体是产生该疾病特征性症状的病理因素。本文描述了一种快速、定量且灵敏的放射免疫测定法，该方法使用经碘化α-银环蛇毒素亲和标记的人乙酰胆碱受体，并利用带有免疫吸附蛋白A的金黄色葡萄球菌。在诊断筛查、评估各种治疗形式、确定受体与抗体相互作用的动力学以及培养的外周血淋巴细胞产生抗受体抗体等方面，该测定法已被证明比双抗体免疫沉淀测定法更有效且更高效。