人IgM、IgG、IgA、IgD和IgE的简单悬滴（20微升）双抗体放射免疫测定法

Simple hanging drop (20 microliter) double antibody radioimmunoassay of human IgM, IgG, IgA, IgD and IgE.

作者信息

Newton C A, Pereira R S, Farrant J

出版信息

J Immunol Methods. 1981;45(1):41-9. doi: 10.1016/0022-1759(81)90092-2.

Abstract

A simple hanging drop radioimmunoassay has been developed for different classes of human immunoglobulin from serum and culture supernatant samples. The immune precipitate was formed in inverted Terasaki plates (20 microliter per well) using 4 aliquots (5 microliter) as follows: (A) a standard or unknown sample; (B) rabbit antisera to the appropriate immunoglobulin class; (C) 125I-labelled human immunoglobulin of the appropriate class; (D) a developing goat-anti rabbit serum. The immune precipitate in the hanging drop was harvested and washed without centrifugation with a simple Terasaki plate harvester. The method gives a great saving both in time and in sample and reagent volumes in comparison with an established double antibody assay in tubes (4 X 100 microliter aliquots).

摘要

已开发出一种简单的悬滴放射免疫分析法，用于检测血清和培养上清液样本中的不同类别人免疫球蛋白。使用4个等分试样（5微升）在倒置的寺崎板（每孔20微升）中形成免疫沉淀物，具体如下：（A）标准品或未知样品；（B）针对相应免疫球蛋白类别的兔抗血清；（C）相应类别的125I标记人免疫球蛋白；（D）显色用的山羊抗兔血清。悬滴中的免疫沉淀物用简单的寺崎板收获器收获并洗涤，无需离心。与已确立的试管双抗体分析法（4个100微升等分试样）相比，该方法在时间、样品和试剂用量方面都节省很多。