一种用于在小鼠模型系统中检测微量Ig类特异性抗病毒抗体的固相微放射免疫测定法。

A solid phase micro-radioimmunoassay to detect minute amounts of Ig class specific anti-viral antibody in a mouse model system.

作者信息

Charlton D, Blandford G

出版信息

J Immunol Methods. 1975 Oct;8(4):319-30. doi: 10.1016/0022-1759(75)90053-8.

Abstract

A simple and rapid micro-radioimmunoassay was developed to detect and quantitate class specific mouse anti-Sendai virus antibodies. Two different 125I-labelled indicator systems were studied. After incubation of test serum with antigen one system used 125I-rabbit anti-mouse IgG (RIA 1) and the second employed rabbit anti-mouse IgG, IgA or IgM followed by 125I-sheep anti-rabbit immunoglobulin reagent (RIA 2). The RIA 2 method was adopted for routine use as it was more sensitive, gave better discrimination between sample and background counts and eliminated the need for several labelled rabbit anti-mouse Ig class specific antisera. The technique was found to be about 100 times more sensitive than conventional HI tests, specific, reliable and economical of reagents and time.

摘要

已开发出一种简单快速的微量放射免疫测定法，用于检测和定量小鼠抗仙台病毒抗体的类别特异性抗体。研究了两种不同的¹²⁵I标记指示系统。将待测血清与抗原孵育后，一种系统使用¹²⁵I标记的兔抗小鼠IgG（放射免疫测定法1），另一种系统先使用兔抗小鼠IgG、IgA或IgM，然后使用¹²⁵I标记的羊抗兔免疫球蛋白试剂（放射免疫测定法2）。放射免疫测定法2因其更灵敏、能更好地区分样品计数与背景计数且无需多种标记的兔抗小鼠Ig类别特异性抗血清而被采用用于常规检测。该技术比传统血凝抑制试验灵敏约100倍，具有特异性、可靠性，且试剂和时间成本低。