Siddell S G, Barthel A, ter Meulen V
J Gen Virol. 1981 Feb;52(Pt 2):235-43. doi: 10.1099/0022-1317-52-2-235.
Coronavirus JHM contains six major proteins, one of which, the 60 000 mol. wt. nucleocapsid protein pp60, is phosphorylated. In JHM-infected cells ip 60K, the intracellular precursor to pp60 is also phosphorylated. Associated with purified JHM virions is a protein kinase which will phosphorylate pp60 and a variety of exogenous substances in vitro. The enzyme has the characteristics of a cyclic nucleotide-independent protein kinase. Both the in vivo reaction and the enzyme activity in vitro transferred the gamma-phosphate of ATP to serine residues on the nucleocapsid protein.
冠状病毒JHM含有六种主要蛋白，其中之一是分子量为60000的核衣壳蛋白pp60，它是磷酸化的。在感染JHM的细胞中，pp60的细胞内前体ip 60K也是磷酸化的。与纯化的JHM病毒粒子相关的是一种蛋白激酶，它在体外能使pp60和多种外源物质磷酸化。该酶具有不依赖环核苷酸的蛋白激酶的特性。体内反应和体外酶活性都将ATP的γ-磷酸基团转移到核衣壳蛋白的丝氨酸残基上。
