Casali P, Sissons J G, Fujinami R S, Oldstone M B
J Gen Virol. 1981 May;54(Pt 1):161-71. doi: 10.1099/0022-1317-54-1-161.
We report a simple method for the isolation of the measles virus glycoproteins, and their subsequent incorporation into artificial lipid bilayers. The two viral glycoproteins, HA and F, were isolated in preparative amounts from disrupted purified virus by lentil lectin affinity chromatography. The proteins were reconstituted into single bilayer lipid vesicles by: (i) exchanging the non-dialysable detergent Nonidet P40 (NP40) for a dialysable one, octylglucoside, while the proteins were immobilized on the lectin column and (ii) co-dialysis of the eluted glycoproteins in octylglucoside with phosphatidylcholine. The resultant 'virosomes' had visible 'spikes' and possessed haemagglutinating activity. These measles virosomes should provide a useful reagent for studying immune responses to measles virus, independent of the immunosuppressive effects of the whole virus.
我们报道了一种分离麻疹病毒糖蛋白并将其随后整合到人工脂质双层中的简单方法。通过扁豆凝集素亲和层析从裂解的纯化病毒中以制备量分离出两种病毒糖蛋白，即血凝素（HA）和融合蛋白（F）。通过以下方式将这些蛋白质重构到单层脂质囊泡中：（i）在蛋白质固定在凝集素柱上时，将不可透析的去污剂诺乃洗涤剂P40（NP40）换成可透析的辛基葡糖苷，以及（ii）将洗脱的糖蛋白在辛基葡糖苷中与磷脂酰胆碱进行共透析。所得的“病毒体”具有可见的“刺突”并具有血凝活性。这些麻疹病毒体应为研究针对麻疹病毒的免疫反应提供一种有用的试剂，而不受整个病毒免疫抑制作用的影响。
