Amacher D E, Paillet S C, Elliott J A
Mutat Res. 1981 Aug;89(4):311-20. doi: 10.1016/0165-1218(81)90112-9.
Direct treatment of L5178Y mouse lymphoma TK+/- cells with N-acetyl-2-aminofluorene (AAF) from two commercial sources produced small, but reproducible increases in mutant frequency over background in the absence of exogenous microsomal enzymes. Unlike most direct-acting mutagens which typically produce regular, dose-dependent increases in mutant frequency; AAF treatment caused very slight dose-related increases or a saturation phenomenon which could be overcome by increased exposure time. Direct mutagenicity following prolonged (24h) exposure was confirmed when a third highly purified (99.9%) AAF sample was tested. Microsomal enzyme analyses of disrupted L5178Y cell preparations revealed negligible benzo[a]pyrene hydroxylase but measurable AAF-N-hydroxylase activity. These data demonstrate that L5178Y mouse lymphoma cells are capable of limited metabolism of AAF to an active mutagen.
用来自两个商业来源的N-乙酰-2-氨基芴（AAF）直接处理L5178Y小鼠淋巴瘤TK+/-细胞，在没有外源性微粒体酶的情况下，突变频率比背景值有小但可重复的增加。与大多数典型地产生规则的、剂量依赖性突变频率增加的直接作用诱变剂不同；AAF处理导致非常轻微的剂量相关增加或饱和现象，这可以通过延长暴露时间来克服。当测试第三个高度纯化（99.9%）的AAF样品时，证实了长时间（24小时）暴露后的直接诱变性。对破碎的L5178Y细胞制剂进行的微粒体酶分析显示，苯并[a]芘羟化酶可忽略不计，但可测量到AAF-N-羟化酶活性。这些数据表明，L5178Y小鼠淋巴瘤细胞能够将AAF有限地代谢为活性诱变剂。
