Tong C, Brat S V, Williams G M
Mutat Res. 1981 Nov;91(6):467-73. doi: 10.1016/0165-7992(81)90054-3.
Adult rat-liver epithelial cell lines possess intrinsic metabolic capability for the biotransformation of xenobiotics and thus, are sensitive to a broad spectrum of mutagens/carcinogens in a mutagenesis assay at the hypoxanthine-guanine phosphoribosyl transferase locus. To provide another end-point of biological significance in these lines, we have investigated the application of adult rat-liver epithelial cell line 18 in a sister-chromatid exchange assay. Significant dose-dependent increases in the sister-chromatid exchange frequency occurred when liver cells were exposed to benzo[a]pyrene and 7,12-dimethylbenz[a]anthracene. A weak but positive response was elicited by benz[a]anthracene. The present observations thus confirm the capacity of these cells to generate genotoxic metabolites from activation-dependent mutagens/carcinogens and indicate a relationship between the production of mutations and sister-chromatid exchanges by polycyclic aromatic hydrocarbons.
成年大鼠肝脏上皮细胞系具有对外源化学物质进行生物转化的内在代谢能力，因此，在次黄嘌呤 - 鸟嘌呤磷酸核糖转移酶基因座的诱变试验中，它们对多种诱变剂/致癌物敏感。为了在这些细胞系中提供另一个具有生物学意义的终点指标，我们研究了成年大鼠肝脏上皮细胞系18在姐妹染色单体交换试验中的应用。当肝细胞暴露于苯并[a]芘和7,12 - 二甲基苯并[a]蒽时，姐妹染色单体交换频率出现了显著的剂量依赖性增加。苯并[a]蒽引发了微弱但呈阳性的反应。因此，目前的观察结果证实了这些细胞从依赖激活的诱变剂/致癌物产生遗传毒性代谢物的能力，并表明多环芳烃产生的突变与姐妹染色单体交换之间存在关联。
