De Salvia R, Meschini R, Fiore M, Polani S, Palitti F, Carluccio M A, Turchi G
Centro di Genetica Evoluzionistica C.N.R., Univ. La Sapienza, Rome, Italy.
Mutat Res. 1988 Feb;207(2):69-75. doi: 10.1016/0165-7992(88)90044-9.
An epithelial cell strain has been established from the livers of male Chinese hamsters (CHEL cells). These cells, which proliferate in culture and retain their metabolic enzymatic activities during several subcultures, were used in a sister-chromatid exchange assay to evaluate the effectiveness of polycyclic aromatic hydrocarbons (PAHs), aflatoxin B1 (AFB1) and cyclophosphamide (CP). The results obtained demonstrate that CHEL cells are metabolically competent to activate different classes of procarcinogens into biologically active metabolites. Moreover, they showed a selective capacity to discriminate chemical carcinogens from noncarcinogens. Thus, the CHEL cell system appears to be a promising alternative to the short-term tests that include cell-free rodent liver homogenate to evaluate new promutagens and/or procarcinogens.
已从雄性中国仓鼠的肝脏中建立了一种上皮细胞系（CHEL细胞）。这些细胞在培养中增殖，并在多次传代培养过程中保持其代谢酶活性，被用于姐妹染色单体交换试验，以评估多环芳烃（PAHs）、黄曲霉毒素B1（AFB1）和环磷酰胺（CP）的有效性。获得的结果表明，CHEL细胞在代谢方面有能力将不同种类的前致癌物激活为具有生物活性的代谢物。此外，它们表现出从非致癌物中区分化学致癌物的选择性能力。因此，CHEL细胞系统似乎是一种有前景的替代方法，可替代包括无细胞啮齿动物肝脏匀浆在内的短期试验，用于评估新的促突变剂和/或前致癌物。
