Krajcovicová M, Dibák O, Bucko A
Nahrung. 1981;25(7):647-54. doi: 10.1002/food.19810250707.
Low-protein (5%) and high-protein (40%) diets as well as low-fat (5%) and high-fat (40%) diets were fed to weanling rats in a 14-day experiment, the standard diet containing 10% of protein and 10% of fat. The net protein utilization of casein, the liver protein utilization and the activity of the key enzyme of gluconeogenesis in the liver, phosphoenol pyruvate carboxykinase, were determined. The utilization of proteins by the organism was adversely affected by a high intake of proteins and also of fats. This fact and the observed stimulation of gluconeogenesis testify to the physiological and economical unsuitableness of diets containing excessive amounts of proteins and fats. A low fat intake was less detrimental to the utilization of proteins than a high fat intake; a low intake of proteins seemed to improve the utilization, but other parameters evidenced that it is not sufficient. The application of diets with closely graded contents in wide ranges for various times will yield biochemical indicators being of importance as supplementary factors in the determination of the physiological value of nutrients under different conditions.
在一项为期14天的实验中，给断奶大鼠喂食低蛋白（5%）和高蛋白（40%）饮食以及低脂肪（5%）和高脂肪（40%）饮食，标准饮食含10%的蛋白质和10%的脂肪。测定了酪蛋白的净蛋白质利用率、肝脏蛋白质利用率以及肝脏中糖异生关键酶磷酸烯醇式丙酮酸羧激酶的活性。机体对蛋白质的利用受到高蛋白和高脂肪摄入量的不利影响。这一事实以及观察到的糖异生刺激证明了含有过量蛋白质和脂肪的饮食在生理和经济上的不合适性。低脂肪摄入量对蛋白质利用的损害小于高脂肪摄入量；低蛋白质摄入量似乎能提高利用率，但其他参数表明这还不够。在不同时间段应用蛋白质和脂肪含量在很宽范围内紧密分级的饮食，将产生作为补充因素的生化指标，对在不同条件下确定营养素的生理价值具有重要意义。
