Calabrese S, Giansante C, Burigana F, Feruglio F S
Minerva Med. 1981 Sep 19;72(34):2255-60.
We studied 91 children, distributed, as follows, in four groups: Group A, including 24 children (average age 11.4 +/- 0.7) with juvenile onset diabetes (duration less than 4 years), without any clinically apparent vascular disorder. Group B: 22 matched controls (average age 11.5 +/- 0.53). Group C: 25 newborns (average age 3.7 +/- 0.3) of diabetic mothers (B Group in P. White classification). Group D: 20 matched controls (average age 2.8 +/- 0.3). All were tested for total, HDL, LDL-cholesterol, triglycerides, fasting blood sugar, systolic and diastolic blood pressure. In all the subjects we performed: -- PF4 (by H.T.C.H., according O'Brien et al., 1975) on PPP and on PRP (300,000-350,000 platelets/microliters) after platelet aggregation with 4.0 10(-6)M epinephrine (Born's method). -- PF3 (b a Stypven time) on frozen-thawed (three times) PRP and on PRP after p.a. with 4.0 10(-6)M epinephrine. No statistically significant difference was noted in PF3 values between A-C groups and their respective control groups (B-D). PF4 was more represented on diabetic's PPP (group A), whilst no difference was noted after aggregation. This increase suggests in early juvenile onset diabetes, without any clinically apparent disease, an "in vivo" platelet activation, which may be important in thromboembolic disorders of diabetics.
我们研究了91名儿童，分为以下四组：A组，包括24名儿童（平均年龄11.4±0.7岁），患有青少年期发病的糖尿病（病程少于4年），无任何临床明显的血管疾病。B组：22名匹配的对照组儿童（平均年龄11.5±0.53岁）。C组：25名糖尿病母亲的新生儿（平均年龄3.7±0.3岁）（怀特分类法中的B组）。D组：20名匹配的对照组儿童（平均年龄2.8±0.3岁）。对所有儿童均进行了总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、甘油三酯、空腹血糖、收缩压和舒张压的检测。在所有受试者中，我们进行了以下操作：——在血小板用4.0×10⁻⁶M肾上腺素聚集（博恩方法）后，在血小板贫乏血浆（PPP）和富血小板血浆（PRP）（300,000 - 350,000个血小板/微升）上按照奥布赖恩等人1975年的方法用高氯酸（H.T.C.H.）检测PF4。——在冻融（三次）的PRP和用4.0×10⁻⁶M肾上腺素聚集后的PRP上通过蛇毒时间检测PF3。A - C组与其各自的对照组（B - D组）之间在PF3值上未发现统计学上的显著差异。PF4在糖尿病患者的PPP（A组）中表现更为明显，而聚集后未发现差异。这种增加表明在青少年期发病的早期糖尿病中，在无任何临床明显疾病的情况下，存在“体内”血小板激活，这可能在糖尿病患者的血栓栓塞性疾病中具有重要意义。
