Breddin K, Grun H, Krzywanek H J, Schremmer W P
Thromb Haemost. 1976 Jun 30;35(3):669-91.
A new measuring device was developed for the study of "spontaneous" aggregating activity of thrombocytes. In the photometric platelet aggregation test (PAT III) 0.6 ml of platelet-rich plasma (PRP) are rotated in a disc-shaped cuvette at 20 rpm and 37 degrees C. Changes in optical density of PRP which are induced by the formation of platelet aggregates are continuously registered using a chart recorder. PAT III was developed for the detection of enhanced platelet aggregation, indicating a risk of thrombosis and thromboembolic complications. In 146 healthy individuals a certain percentage showed slight primary aggregation (alpha1) which in some cases was followed by marked aggregation (alpha2) at a certain time (Tr) after the beginning of rotation. The percentage of individuals showing alpha2 increased with age. An increase of plasma pH in the rotating sample, which was caused by diffusion of CO2, was an important conditioning factor for aggregation. The test results depended on the platelet count in PRP. Aggregation curves were suppressed by admixture of erythrocytes and lipid turbidity. The tendency of platelets to aggregate increased within 60-90 min following blood sampling. During this period the interval to the onset of aggregation (Tr) became shorter and the maximum aggregation speed (alpha 2) increased with time. PAT III yielded reproducible results when it was carried out more than 60 min after blood drawing. In a group of 327 diabetic patients "spontaneous" aggregation occurred more frequently in all age groups as compared with the controls. Additional equipment was available for the registration of ADP-, collagen-, or epinephrine-induced aggregation similar to Born's and O'Brien's method. The device can easily be mounted on an Eppendorf photometer without further alterations.
为研究血小板的“自发”聚集活性，开发了一种新的测量装置。在光度血小板聚集试验（PAT III）中，0.6毫升富血小板血浆（PRP）在盘形比色皿中于20转/分钟和37摄氏度下旋转。使用图表记录仪连续记录由血小板聚集体形成引起的PRP光密度变化。PAT III用于检测增强的血小板聚集，提示血栓形成和血栓栓塞并发症的风险。在146名健康个体中，一定比例的人显示出轻微的初始聚集（α1），在某些情况下，在旋转开始后的特定时间（Tr）会出现明显的聚集（α2）。显示α2的个体百分比随年龄增加。旋转样本中由于二氧化碳扩散导致的血浆pH值升高是聚集的一个重要调节因素。测试结果取决于PRP中的血小板计数。红细胞和脂质浊度的混入可抑制聚集曲线。采血后60 - 90分钟内血小板聚集的趋势增加。在此期间，聚集开始的间隔时间（Tr）变短，最大聚集速度（α2）随时间增加。在采血后60分钟以上进行PAT III时，可得到可重复的结果。在一组327名糖尿病患者中，与对照组相比，所有年龄组中“自发”聚集更为频繁。还有额外的设备可用于记录类似于博恩（Born）和奥布赖恩（O'Brien）方法的由二磷酸腺苷（ADP）、胶原或肾上腺素诱导的聚集。该装置可轻松安装在艾本德（Eppendorf）光度计上，无需进一步改装。
