Campbell C E, Gravel R A, Worton R G
Somatic Cell Genet. 1981 Sep;7(5):535-46. doi: 10.1007/BF01549657.
Stable mutants resistant to the toxic anion chromate have been isolated from a variety of Chinese hamster cell lines. The mechanism of chromate toxicity is not known, but it must involve internalization via the sulfate transport pathway. All mutant lines had a defective sulfate transport system, showing a 10-fold reduction in the rate of uptake of radioactive sulfate into the cell. The chromate resistance phenotype in CHO cell mutants behave recessively in somatic cell hybrids; in other cell lines the Chr(r) phenotype was partially expressed (codominant) in cell hybrids. Complementation analysis in cell hybrids between 18 different mutant pairs failed to reveal any complementation, indicating that chromate selects mutants primarily, if not exclusively, at a single gene locus.
已从多种中国仓鼠细胞系中分离出对有毒阴离子铬酸盐具有抗性的稳定突变体。铬酸盐毒性的机制尚不清楚，但它必定涉及通过硫酸盐转运途径的内化过程。所有突变细胞系的硫酸盐转运系统均存在缺陷，放射性硫酸盐进入细胞的摄取速率降低了10倍。CHO细胞突变体中的铬酸盐抗性表型在体细胞杂种中呈隐性表现；在其他细胞系中，Chr(r)表型在细胞杂种中部分表达（共显性）。对18对不同突变体之间的细胞杂种进行互补分析，未发现任何互补现象，这表明铬酸盐主要（如果不是唯一）在单个基因位点选择突变体。
