Dana S, Wasmuth J J
Mol Cell Biol. 1982 Oct;2(10):1220-8. doi: 10.1128/mcb.2.10.1220-1228.1982.
Chinese hamster-human interspecific hybrid cells, which contain human chromosome 5 and express four genes linked on that chromosome, were subjected to selective conditions requiring them to retain one of the four linked genes, leuS (encoding leucyl-tRNA synthetase), but lose another, either emtB (encoding ribosomal protein S14) or chr. Cytogenetic and biochemical analyses of spontaneous segregants isolated by using these unique selective pressures have enabled us to determine the order and regional location of the leuS, hexB, emtB, and chr genes on human chromosome 5. These segregants arise primarily by terminal deletions of various portions of the long arm of chromosome 5. Our results indicate that the order of at least three of these genes is the same on human chromosome 5 and Chinese hamster chromosome 2. Thus, there appears to be extensive homology between Chinese hamster chromosome 2 and human chromosome 5, which represents an extreme example of the conservation of gene organization between very divergent mammalian species. In addition, these hybrids and selective conditions provide a very simple and quantitative means to assess the potency of various agents suspected of inducing gross chromosomal damage.
中国仓鼠 - 人类种间杂交细胞含有人类5号染色体并表达该染色体上连锁的四个基因，这些细胞在选择性条件下需要保留四个连锁基因之一leuS（编码亮氨酰 - tRNA合成酶），但丢失另一个基因，即emtB（编码核糖体蛋白S14）或chr。通过使用这些独特的选择压力分离出的自发分离株的细胞遗传学和生化分析，使我们能够确定leuS、hexB、emtB和chr基因在人类5号染色体上的顺序和区域位置。这些分离株主要通过5号染色体长臂不同部分的末端缺失产生。我们的结果表明，这些基因中至少有三个在人类5号染色体和中国仓鼠2号染色体上的顺序相同。因此，中国仓鼠2号染色体和人类5号染色体之间似乎存在广泛的同源性，这代表了非常不同的哺乳动物物种之间基因组织保守性的一个极端例子。此外，这些杂种和选择条件提供了一种非常简单和定量的方法来评估各种疑似诱导染色体严重损伤的试剂的效力。
