Mukazhanova G N, Siniakov M S, Kharitonenkov I G
Vopr Virusol. 1981 May-Jun(3):275-80.
Gel filtration and ion-exchange chromatography were used to isolate a fraction with the highest proteolytic activity and virtually lacking glycosidases from a commercial bromeline preparation ("Serva", grade B) showing, in addition to proteolytic, also glycosidase activity. The utilization of purified bromeline allows isolation of hemagglutinin without residual neuraminidase activity from influenza virus of various serotypes (H1, H1, and H3). The results are discussed on the basis of an assumption of analogous localization but different conformation accessibility of bromeline-sensitive parts of the light chains of influenza viruses of different subtypes, and a possible role of the carbohydrate component of surface glycoproteins, hemagglutinin and neuraminidase of influenza virus virions.
使用凝胶过滤和离子交换色谱法从一种商业菠萝蛋白酶制剂（“Serva”，B级）中分离出具有最高蛋白水解活性且几乎没有糖苷酶活性的部分，该制剂除了具有蛋白水解活性外，还具有糖苷酶活性。利用纯化的菠萝蛋白酶可从各种血清型（H1、H1和H3）的流感病毒中分离出没有残留神经氨酸酶活性的血凝素。基于不同亚型流感病毒轻链中菠萝蛋白酶敏感部分类似定位但构象可及性不同的假设，以及流感病毒颗粒表面糖蛋白、血凝素和神经氨酸酶的碳水化合物成分的可能作用，对结果进行了讨论。
