[Isolation of influenza virus hemagglutinin and its separation into subunits by a stage-by-stage scheme for viral protein fractionation].

The virus envelope protein, hemagglutinin, recovered by DTAB extraction of influenza virions, was purified to a high degree by the gel filtration high-performance liquid chromatography (GF HPLC). Then hemagglutinin was reduced and alkylated with iodoacetamide and the subunits were resolved by GF HPLC on the columns Spherogel TSK G 2000 SW and 3000 SW (600 X 7.5 mm ID).