Mochly-Rosen D, Fuchs S
Biochemistry. 1981 Sep 29;20(20):5920-4. doi: 10.1021/bi00523a041.
We have isolated 32 hybridoma cell lines producing monoclonal antibodies against the acetylcholine receptor from Torpedo californica. One of these lines, designated 5.5.G.12, secretes antibodies which are directed against the cholinergic binding site of the acetylcholine receptor. This specific antibody blocked the binding of alpha-bungarotoxin to the acetylcholine receptor. The binding of monoclonal antibody 5.5.G.12 to acetylcholine receptor was inhibited by alpha-neurotoxins and by other cholinergic ligands in accordance with their affinities to the nicotinic acetylcholine receptor. None of the other monoclonal antibodies obtained inhibited the binding of alpha-bungarotoxin to acetylcholine receptor, nor was their binding to the acetylcholine receptor inhibited by cholinergic ligands. The monoclonal antibody elicited against the binding site of Torpedo acetylcholine receptor bound also to acetylcholine receptors of various species and organs, demonstrating the wide structural homology between the cholinergic sites of various acetylcholine receptors.
我们从加州电鳐中分离出了32种产生抗乙酰胆碱受体单克隆抗体的杂交瘤细胞系。其中一个细胞系，命名为5.5.G.12，分泌的抗体针对乙酰胆碱受体的胆碱能结合位点。这种特异性抗体阻断了α-银环蛇毒素与乙酰胆碱受体的结合。单克隆抗体5.5.G.12与乙酰胆碱受体的结合受到α-神经毒素和其他胆碱能配体的抑制，这与它们对烟碱型乙酰胆碱受体的亲和力一致。获得的其他单克隆抗体均未抑制α-银环蛇毒素与乙酰胆碱受体的结合，它们与乙酰胆碱受体的结合也未受到胆碱能配体的抑制。针对电鳐乙酰胆碱受体结合位点产生的单克隆抗体也与各种物种和器官的乙酰胆碱受体结合，这表明各种乙酰胆碱受体的胆碱能位点之间存在广泛的结构同源性。
