水杨酸对大鼠骨骼肌线粒体棕榈酸氧化的刺激作用。

Salicylic acid stimulation of palmitic acid oxidation by rat skeletal muscle mitochondria.

作者信息

Jones R E, Askew E W, Hecker A L, Hofeldt F D

出版信息

Biochim Biophys Acta. 1981 Oct 23;666(1):120-6. doi: 10.1016/0005-2760(81)90097-7.

Abstract

The effects of salicylic acid on palmitic acid oxidation were studied using rat skeletal muscle mitochondria. Salicylic acid, in concentrations that exerted no effect on mitochondrial coupling (0.1 mM), significantly stimulated mitochondrial palmitic acid oxidation, with maximal stimulation occurring at subsaturating concentrations of substrate. In the same preparation, salicylate had no effect on the oxidation of palmitoylcarnitine or palmitoyl-CoA. Salicylate appears to augment the initial step of palmitic acid oxidation by lowering the apparent Michaelis constant (Km) of long chain fatty acid: CoASH ligase (AMP) (EC 6.2.1.3) for palmitic acid.

摘要

使用大鼠骨骼肌线粒体研究了水杨酸对棕榈酸氧化的影响。水杨酸浓度对线粒体偶联无影响（0.1 mM）时，能显著刺激线粒体棕榈酸氧化，在底物亚饱和浓度时刺激作用最大。在同一制剂中，水杨酸盐对棕榈酰肉碱或棕榈酰辅酶A的氧化无影响。水杨酸盐似乎通过降低长链脂肪酸：辅酶A连接酶（AMP）（EC 6.2.1.3）对棕榈酸的表观米氏常数（Km）来增强棕榈酸氧化的起始步骤。