Giometti C S, Anderson N G
Clin Chem. 1981 Nov;27(11):1918-21.
Proteins from frozen histological sections of human muscle were analyzed by two-dimensional gel electrophoresis. Patterns so obtained were identical to those from whole homogenates of muscle prepared from frozen tissue powders that had much higher protein concentrations. To increase the number of proteins visible on gels of samples low in protein content, the gels were silver stained, or the proteins were labeled with [14C]iodoacetamide before electrophoresis and the gels were fluorographed. The latter method allow use of a single frozen-tissue section for two-dimensional electrophoretic analysis and brings the technique closer to practicable clinical use.
通过二维凝胶电泳分析了来自人类肌肉冷冻组织切片的蛋白质。如此获得的图谱与来自由冷冻组织粉末制备的肌肉全匀浆的图谱相同，后者的蛋白质浓度要高得多。为了增加蛋白质含量低的样品凝胶上可见的蛋白质数量，对凝胶进行银染，或者在电泳前用[14C]碘乙酰胺标记蛋白质，然后对凝胶进行荧光显影。后一种方法允许使用单个冷冻组织切片进行二维电泳分析，使该技术更接近实际临床应用。
