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使用萘酚黄S和二硝基氟苯染色法对细胞核和细胞质蛋白质进行定量细胞化学分析。

Quantitative cytochemistry of nuclear and cytoplasmic proteins using the Naphthol Yellow S and dinitrofluorobenzene staining methods.

作者信息

Tas J, James J

出版信息

Histochem J. 1981 Sep;13(5):711-6. doi: 10.1007/BF01003283.

DOI:10.1007/BF01003283
PMID:7298374
Abstract

The 'total protein staining' of biological specimens with the electrostatically binding Naphthol Yellow S or the covalently binding dinitrofluorobenzene must be interpreted as methods which yield data on the specific amino acid pool of the proteins concerned. Both dyes bind to certain free amino-acid side-chains, giving different dye--protein ratios for various proteins. In the presence of DNA, dinitrofluorobenzene stains all proteins present in cell nuclei, whereas Naphthol Yellow S only stains the majority of the non-histone proteins. When protein staining methods are combined with the Feulgen--Pararosanile (SO2) procedure for DNA, decreased Feulgen--DNA contents were measured in dinitrofluorobenzene-stained isolated nuclei and lymphocytes.

摘要

用静电结合的萘酚黄S或共价结合的二硝基氟苯对生物标本进行“总蛋白染色”，必须理解为这些方法可提供有关相关蛋白质特定氨基酸库的数据。两种染料都与某些游离氨基酸侧链结合，不同蛋白质的染料 - 蛋白质比例不同。在DNA存在的情况下，二硝基氟苯可对细胞核中存在的所有蛋白质进行染色，而萘酚黄S仅对大多数非组蛋白进行染色。当蛋白质染色方法与用于DNA的福尔根 - 副蔷薇苯胺（SO2）程序结合使用时，在二硝基氟苯染色的分离细胞核和淋巴细胞中测量到福尔根 - DNA含量降低。

相似文献

1
Quantitative cytochemistry of nuclear and cytoplasmic proteins using the Naphthol Yellow S and dinitrofluorobenzene staining methods.使用萘酚黄S和二硝基氟苯染色法对细胞核和细胞质蛋白质进行定量细胞化学分析。
Histochem J. 1981 Sep;13(5):711-6. doi: 10.1007/BF01003283.
2
Combined staining procedures for cytophotometry of protein and DNA Feulgen-Naphthol Yellow S and dinitrofluorobenzene-Feulgen.蛋白质和DNA细胞光度测定的联合染色程序：福尔根-萘酚黄S法和二硝基氟苯-福尔根法
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Protein staining methods in quantitative cytochemistry.定量细胞化学中的蛋白质染色方法。
J Microsc. 1980 Aug;119(3):295-311. doi: 10.1111/j.1365-2818.1980.tb04103.x.
4
Quantification of nuclear non-histone proteins by Feulgen--Naphthol Yellow S cytophotometry.用福尔根-萘酚黄S细胞光度法对核非组蛋白进行定量分析。
Histochem J. 1981 Sep;13(5):717-22. doi: 10.1007/BF01003284.
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The use of Light Green and Orange II as quantitative protein stains, and their combination with the Feulgen method for the simultaneous determination of protein and DNA.使用亮绿和橙黄II作为蛋白质定量染色剂，并将它们与福尔根法相结合用于同时测定蛋白质和DNA。
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Dual wavelength scanning cytophotometry (Bicoscan).双波长扫描细胞光度测定法（双扫描）
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Quantitative aspects of the Naphthol Yellow S staining for proteins studied in a model system of polyacrylamide films and in isolated rat liver cells and nuclei.在聚丙烯酰胺薄膜模型系统以及分离的大鼠肝细胞和细胞核中研究的蛋白质萘酚黄S染色的定量方面。
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Adaptation of the Naphthol Yellow S staining for objects with high protein content.萘酚黄S染色法对高蛋白含量物体的适应性。
Histochemistry. 1978 Apr 4;55(3):185-95. doi: 10.1007/BF00495758.

引用本文的文献

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A modification of the amidoblack-TCA-staining for quantitative microspectrometrical determination of proteins in tissue sections.用于组织切片中蛋白质定量显微光谱测定的酰胺黑 - TCA 染色法的一种改良方法。
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2
The use of Light Green and Orange II as quantitative protein stains, and their combination with the Feulgen method for the simultaneous determination of protein and DNA.使用亮绿和橙黄II作为蛋白质定量染色剂，并将它们与福尔根法相结合用于同时测定蛋白质和DNA。
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3
Changes in nuclear and nucleolar protein content during the growth and differentiation of root parenchyma cells in plant species with different DNA-endoreplication dynamics.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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Microspectrophotometric study of the binding of the anionic dye, naphthol yellow S, by tissue sections and by purified proteins.阴离子染料萘酚黄S与组织切片及纯化蛋白质结合的显微分光光度研究。
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Protein staining methods in quantitative cytochemistry.定量细胞化学中的蛋白质染色方法。
具有不同DNA核内复制动态的植物物种中，根薄壁细胞生长和分化过程中核蛋白和核仁蛋白含量的变化
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J Microsc. 1980 Aug;119(3):295-311. doi: 10.1111/j.1365-2818.1980.tb04103.x.
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Combined protein and DNA measurements in plant cells using the dinitrofluorobenzene and feulgen techniques.
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The naphthol yellow S stain for proteins tested in a model system of polyacrylamide films and evaluated for practical use in histochemistry.在聚丙烯酰胺薄膜模型系统中对蛋白质进行萘酚黄S染色，并对其在组织化学中的实际应用进行评估。
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A model system analysis of the parameters in histone staining. I. Alkaline Fast Green.
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Extinction effects in Feulgen-DNA scanning photometry of human lymphocytes.
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Assay for protein by dye binding.
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An evaluation of the Coomassie brillant blue G-250 dye-binding method for quantitative protein determination.考马斯亮蓝G - 250染料结合法用于蛋白质定量测定的评估。
Anal Biochem. 1977 Aug;81(2):478-80. doi: 10.1016/0003-2697(77)90723-0.