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通过同时进行DNA和核蛋白分析来确定细胞学样本中G0期细胞的比例。

Determination of the fraction of G0 cells in cytologic samples by means of simultaneous DNA and nuclear protein analyses.

作者信息

Auer G, Ono J, Caspersson T O

出版信息

Anal Quant Cytol. 1983 Mar;5(1):1-4.

PMID:6846963
Abstract

The ratio of protein to amount of DNA in the nucleus was cytophotometrically determined by means of a combined Feulgen-naphthol yellow S staining procedure. Quiescent normal lymphocytes, bronchial epithelial cells and mammary epithelial cells all showed ratios that were significantly lower than in the proliferating counterparts of the different cell types. Hence, combined determinations of DNA and nuclear protein make it possible to determine the fraction of growth-arrested cells (G0 cells) in cytologic preparations.

摘要

通过福尔根-萘酚黄S联合染色法，用细胞光度测定法测定细胞核中蛋白质与DNA含量的比例。静止的正常淋巴细胞、支气管上皮细胞和乳腺上皮细胞的比例均显著低于不同细胞类型的增殖对应细胞。因此，对DNA和核蛋白进行联合测定能够确定细胞学制剂中生长停滞细胞（G0期细胞）的比例。

相似文献

1
Determination of the fraction of G0 cells in cytologic samples by means of simultaneous DNA and nuclear protein analyses.通过同时进行DNA和核蛋白分析来确定细胞学样本中G0期细胞的比例。
Anal Quant Cytol. 1983 Mar;5(1):1-4.
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The G0 in equilibrium G1 transitions of human lymphocytes as monitored by quantitative 14C-uridine autoradiography and high-resolution image analysis.通过定量14C-尿苷放射自显影和高分辨率图像分析监测人淋巴细胞在平衡状态下G0到G1的转变。
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Changes in nuclear and nucleolar protein content during the growth and differentiation of root parenchyma cells in plant species with different DNA-endoreplication dynamics.具有不同DNA核内复制动态的植物物种中，根薄壁细胞生长和分化过程中核蛋白和核仁蛋白含量的变化
Histochemistry. 1986;85(1):51-6. doi: 10.1007/BF00508653.
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High-resolution 2-dimensional protein mapping of "diploid" and "aneuploid" mammary adenocarcinomas.
“二倍体”和“非整倍体”乳腺腺癌的高分辨率二维蛋白质图谱
Histochemistry. 1986;84(4-6):338-41. doi: 10.1007/BF00482960.
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A cytophotometric analysis of the structure of hypothalamic cell populations in the frog, Rana temporaria (L.), with special reference to seasonal changes in chromatin status.对林蛙（Rana temporaria (L.)）下丘脑细胞群体结构的细胞光度分析，特别关注染色质状态的季节性变化。
Histochemistry. 1988;88(3-6):629-36. doi: 10.1007/BF00570334.