Janecková V, Znojemská S, Pekárek J
J Hyg Epidemiol Microbiol Immunol. 1981;25(3):244-51.
The sensitization properties of a number of chemicals were estimated on experimental models, using white female guinea pigs. Sensitization as effected in four ways a) intradermal application jointly with Freund's complete adjuvants (FCA), b) repeated epicutaneous applications together with FCA intradermally, c) a single intradermal application of the tested substance to the ear lobe, d) a single injection of the substance together with FCA into the paws. The results were evaluated with the skin test (ST) and in vitro with the macrophage migration inhibition test (MIT). The suitability of these methodical procedures was verified and agreement in the positivity of the ST and MIT confirmed. The sensitization effect of the tested substance does not depend on the intensity of skin reaction but on the frequency of positive skin test results in the monitored sample.
使用白色雌性豚鼠，在实验模型上评估了多种化学物质的致敏特性。致敏通过四种方式实现：a）与弗氏完全佐剂（FCA）联合皮内应用；b）皮内注射FCA的同时反复经皮应用；c）将受试物质单次皮内注射到耳垂；d）将受试物质与FCA一起单次注射到爪中。结果通过皮肤试验（ST）进行评估，并在体外通过巨噬细胞迁移抑制试验（MIT）进行评估。验证了这些方法程序的适用性，并确认了ST和MIT阳性结果的一致性。受试物质的致敏作用不取决于皮肤反应的强度，而是取决于监测样本中皮肤试验阳性结果的频率。
