肽聚糖对腹膜巨噬细胞的迁移抑制作用。

Migration inhibition of peritoneal macrophages by peptidoglycan.

作者信息

Bültmann B, Heymer B, Schleifer K H, Seidl H P, Haferkamp O

出版信息

Z Immunitatsforsch Exp Klin Immunol. 1975 Jul;149(2-4):289-94.

Abstract

Previous studies have shown that peptidoglycans from group A and B streptococci inhibit the migration of peritoneal exudate cells from non-sensitized rats and guinea pigs. In the present studies peptidoglycans from S. aureus and S. epidermidis were found to inhibit the migration of cells to the same extent as group A streptococcal peptidoglycan. In contrast, HSA-pentapeptide, an immunologically active synthetic analog of the peptide moiety of peptidoglycan which is free of the intrinsic toxicity of naturally occurring peptidoglycans, did not induce migration inhibition of peritoneal exudate cells from non-sensitized guinea pigs. Sensitization of animals with 200 mug HSA-pentapeptide emulsified in incomplete Freund's adjuvant significantly reduced the inhibitory effect of streptococcal and staphylococcal peptidoglycan; HSA-pentapeptide again showed no activity. However, when HSA-pentapeptide was tested against cells from animals sensitized with 200 mug HSA-pentapeptide incorporated in complete Freund's adjuvant, a strong inhibition of migration was evident. Skin tests performed in these animals, in contrast to the dermonecrotic reaction elicited by streptococcal or staphylococcal peptidoglycan, revealed a characteristic delay hypersensitivity to HSA-pentapeptide.

摘要

先前的研究表明，A组和B组链球菌的肽聚糖可抑制未致敏大鼠和豚鼠腹腔渗出细胞的迁移。在本研究中，发现金黄色葡萄球菌和表皮葡萄球菌的肽聚糖对细胞迁移的抑制程度与A组链球菌肽聚糖相同。相比之下，HSA-五肽是一种肽聚糖肽部分的免疫活性合成类似物，无天然肽聚糖的内在毒性，它不会诱导未致敏豚鼠腹腔渗出细胞的迁移抑制。用不完全弗氏佐剂乳化的200μg HSA-五肽对动物进行致敏，可显著降低链球菌和葡萄球菌肽聚糖的抑制作用；HSA-五肽再次显示无活性。然而，当用完全弗氏佐剂中掺入的200μg HSA-五肽对动物进行致敏后，用HSA-五肽检测其细胞时，明显出现了对迁移的强烈抑制。与链球菌或葡萄球菌肽聚糖引起的皮肤坏死反应相反，在这些动物身上进行的皮肤试验显示出对HSA-五肽的特征性迟发型超敏反应。