Chinault D N, Thompson H A, Gangemi J D
J Gen Virol. 1981 Jul;55(Pt 1):213-7. doi: 10.1099/0022-1317-55-1-213.
Pichinde virus preparations were investigated for ribosomal components and associated activities. After detergent treatment and fractionation, viral components were assayed for polypeptide synthesis, elongation of nascent polypeptide chains, and mRNA activity. It was demonstrated that these subviral particles could synthesize polypeptides when exogenous mRNA template, aminoacyl--tRNAs, translation factors, GTP and appropriate cations were added. Undisrupted, whole virions could not synthesize polypeptide; disruption and subsequent centrifugation of subviral ribosomes was a prerequisite for the biosynthetic activity described.
对皮钦德病毒制剂进行了核糖体成分及相关活性的研究。经过去污剂处理和分级分离后，对病毒成分进行了多肽合成、新生多肽链延伸及mRNA活性的检测。结果表明，当加入外源mRNA模板、氨酰-tRNA、翻译因子、GTP和适当的阳离子时，这些亚病毒颗粒能够合成多肽。完整的病毒粒子不能合成多肽；亚病毒核糖体的破坏及随后的离心是所述生物合成活性的先决条件。
