Bonney R C, Moore H D, Jones D M
J Reprod Fertil. 1981 Nov;63(2):523-31. doi: 10.1530/jrf.0.0630523.
Plasma levels of oestradiol and progesterone in 3 pumas during oestrus and artificially induced pregnancy and pseudopregnancy were determined by radioimmunoassay. During oestrus, basal levels of oestradiol (5-30 pg/ml) were interrupted by surges of 30-375 pg/ml at intervals of 17-25 days. Considerable variation occurred between and within animals. Periods of oestrus and follicular development were confirmed by laparoscopy and vaginal smear patterns. Absence of an increase in plasma progesterone following elevated levels of oestradiol and failure to observe corpora lutea in the ovary indicated that ovulation was probably not spontaneous. Plasma oestradiol values rose dramatically in response to PMSG but were low during pseudopregnancy until the return to oestrus. During pregnancy surges of oestradiol occurred at mid-term and immediately before parturition. Ovulation, confirmed by laparoscopy, occurred 24-48 h after hCG and progesterone levels (usually less than 2 ng/ml) then increased to reach 150-300 ng/ml on Days 24-28. In the pseudopregnant animals progesterone concentrations had returned to baseline by Days 45-50 but during pregnancy progesterone remained elevated, declining only gradually to basal values by Day 85. A minor peak of progesterone, coincident with the pre-partum oestradiol surge, occurred on Day 87, 1 week before parturition on Day 95.
通过放射免疫分析法测定了3只美洲狮在发情期、人工诱导怀孕和假孕期间的血浆雌二醇和孕酮水平。在发情期，雌二醇的基础水平（5 - 30 pg/ml）会被17 - 25天间隔出现的30 - 375 pg/ml的峰值打断。不同动物之间以及同一动物个体内均存在相当大的差异。通过腹腔镜检查和阴道涂片模式确认了发情期和卵泡发育阶段。雌二醇水平升高后血浆孕酮未增加，且在卵巢中未观察到黄体，这表明排卵可能不是自发的。血浆雌二醇值对孕马血清促性腺激素（PMSG）有显著反应而升高，但在假孕期间一直较低，直到恢复到发情期。在怀孕期间，雌二醇在中期和临产前会出现峰值。经腹腔镜检查确认，排卵发生在人绒毛膜促性腺激素（hCG）注射后24 - 48小时，孕酮水平（通常低于2 ng/ml）随后升高，在第24 - 28天达到150 - 300 ng/ml。在假孕动物中，孕酮浓度在第45 - 50天恢复到基线水平，但在怀孕期间孕酮一直保持升高，直到第85天才逐渐降至基础值。在第87天，即分娩前95天的前1周，出现了一个与产前雌二醇峰值同时出现的小孕酮峰值。
