Vander Salm T J, Pape L A, Price J, Burke M
J Thorac Cardiovasc Surg. 1981 Nov;82(5):768-72.
Four groups of 12 dogs each had ligation of the left anterior descending coronary artery (LAD) with subsequent release of the ligature and confirmed reperfusion. After 24 hours of reperfusion, the hearts were removed. sliced at 1 cm intervals along the transverse axis, and stained with triphenyltetrazolium chloride. Measurements of the infarct size as a percentage of the left ventricular area (I) and of hemorrhage as a percentage of infarct size (H) were made. The duration of ligation was 3 hours in Group 1, 6 hours in Group II, 18 hours in Group III, and 30 hours in Group IV. No significant difference in infarct size was found among the groups. Percent hemorrhage was 25.2% of infarct area in Group I, 28.3% in Group II, 18.1% in Group II, and 0.7% in Group IV. If reperfusion hemorrhage into an acute myocardial infarct is deleterious, these data suggest than danger to be decreased at 18 hours and absent at 30 hours after acute coronary occlusion.
将四组，每组12只狗，进行左冠状动脉前降支（LAD）结扎，随后松开结扎线并确认再灌注。再灌注24小时后，取出心脏，沿横轴每隔1厘米切片，并用氯化三苯基四氮唑染色。测量梗死面积占左心室面积的百分比（I）以及出血面积占梗死面积的百分比（H）。第一组结扎持续时间为3小时，第二组为6小时，第三组为18小时，第四组为30小时。各组之间梗死面积无显著差异。第一组出血占梗死面积的百分比为25.2%，第二组为28.3%，第三组为18.1%，第四组为0.7%。如果急性心肌梗死时再灌注出血有害，这些数据表明，急性冠状动脉闭塞后18小时危险降低，30小时则无危险。
