家蚕绒毛膜基因的组织，一个多基因家族。I. 与2号染色体连锁的证据。
Organization of the chorion genes of Bombyx mori, a multigene family. I. Evidence for linkage to chromosome 2.
作者信息
Goldsmith M R, Basehoar G
出版信息
Genetics. 1978 Oct;90(2):291-310. doi: 10.1093/genetics/90.2.291.
The chorion genes of silkmoths comprise a multigene family that codes for 50 or more highly specialized structural proteins found in the eggshell. A detailed study of the chromosomal organization of these genes was initiated, using inbred stocks of Bombyx mori as a source of electrophoretic variants for genetic markers. Chorion protein patterns were screened on thin-slab polyacrylamide isoelectric focusing gels. A wide range of polymorphism was observed between stocks. However, isoelectric focusing patterns obtained within a stock were nearly homogeneous, indicating that inbreeding has produced a high degree of homozygosis. Testcrosses were carried out to examine the linkage relationships between electrophoretic markers in four inbred stocks. One race (C108) was selected as a standard against which to compare the inheritance of the variants found in the other three stocks. Chorion markers behaved like codominant Mendelian traits in F, crosses. A total of 15 out of 16 C108 markers cosegregated in subsequent testcrosses, indicating that they are linked. These genes were mapped to the second chromosome, using markers Gr and Y.
蚕蛾的绒毛膜基因构成了一个多基因家族，该家族编码蛋壳中发现的50种或更多高度专业化的结构蛋白。利用家蚕的近交品系作为遗传标记的电泳变异体来源，对这些基因的染色体组织进行了详细研究。在薄平板聚丙烯酰胺等电聚焦凝胶上筛选绒毛膜蛋白模式。在不同品系之间观察到广泛的多态性。然而，在一个品系内获得的等电聚焦模式几乎是同质的，这表明近亲繁殖产生了高度的纯合性。进行测交以检验四个近交品系中电泳标记之间的连锁关系。选择一个品系（C108）作为标准，用以比较其他三个品系中发现的变异体的遗传情况。绒毛膜标记在F1杂交中表现为共显性孟德尔性状。在随后的测交中，16个C108标记中有15个共分离，表明它们是连锁的。利用标记Gr和Y将这些基因定位到第二条染色体上。
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