Balint J A, Kyriakides E C, Beeler D A
Lipids. 1981 Oct;16(10):767-70. doi: 10.1007/BF02535348.
lung slices from rats fed a fat-free diet supplemented with safflower oil (control) or tripalmitoylglycerol (essential fatty acid [EFA]-deficient) were incubated with [14C]acetate, [14C]palmitate, or [14C]stearate. Of the 14C recovered in phospholipids after incubation with [14C]acetate, more than 87% was in 16-carbon fatty acids. Desaturation, as assayed by the percentage of radioactivity in monoenoates in phospholipid fatty acids, was generally double in EFA-deficient slices compared to control slices, regardless of substrate. Desaturation was significantly greater in slices incubated with with acetate or octanoate compared to palmitate, indicating that endogenously synthesized palmitate was desaturated more actively than that derived from an exogenous source.
将喂食补充了红花油（对照）或三棕榈酸甘油酯（缺乏必需脂肪酸[EFA]）的无脂饮食的大鼠的肺切片与[14C]乙酸盐、[14C]棕榈酸盐或[14C]硬脂酸盐一起孵育。在用[14C]乙酸盐孵育后，在磷脂中回收的14C中，超过87%存在于16碳脂肪酸中。通过磷脂脂肪酸中单烯酸酯的放射性百分比测定的去饱和作用，无论底物如何，与对照切片相比，EFA缺乏的切片中通常是对照切片的两倍。与棕榈酸盐相比，用乙酸盐或辛酸孵育的切片中的去饱和作用明显更大，这表明内源性合成的棕榈酸盐比外源性来源的棕榈酸盐去饱和更活跃。
