Chida N, Adams F H
J Lipid Res. 1967 Jul;8(4):335-41.
Incorporation of acetate-1-(14)C into phospholipids and fatty acids by lung slices from fetal and newborn lambs and from ewes was studied in vitro. The distribution of radioactivity in the fatty acids of neutral lipids, phospholipids, and lecithin was determined. Acetate-1-(14)C was incorporated into myristic, palmitic, and C(18) fatty acids. Of the lecithin fatty acids, myristic and palmitic were the major radioactive fatty acids. The results indicate that the lung of fetal lambs is able to synthesize lecithin containing saturated fatty acids, a major constituent of pulmonary surfactant. A marked increase in the rate of acetate incorporation into lecithin was observed during maturation, and these rates were higher than those obtained in the ewes. A possible relationship between developmental changes in lecithin biosynthesis and pulmonary surfactant is discussed.
对来自胎儿和新生羔羊以及母羊的肺切片在体外将乙酸盐 -1-（14）C 掺入磷脂和脂肪酸的情况进行了研究。测定了放射性在中性脂质、磷脂和卵磷脂脂肪酸中的分布。乙酸盐 -1-（14）C 被掺入肉豆蔻酸、棕榈酸和 C18 脂肪酸中。在卵磷脂脂肪酸中，肉豆蔻酸和棕榈酸是主要的放射性脂肪酸。结果表明，胎儿羔羊的肺能够合成含有饱和脂肪酸的卵磷脂，而饱和脂肪酸是肺表面活性物质的主要成分。在成熟过程中观察到乙酸盐掺入卵磷脂的速率显著增加，并且这些速率高于在母羊中获得的速率。讨论了卵磷脂生物合成的发育变化与肺表面活性物质之间可能的关系。
