枯草芽孢杆菌非起始甲硫氨酸转运核糖核酸的核苷酸序列。
Nucleotide sequence of non-initiator methionine tRNA from Bacillus subtilis.
作者信息
Yamada Y, Ishikura H
出版信息
Nucleic Acids Res. 1980 Oct 10;8(19):4517-20. doi: 10.1093/nar/8.19.4517.
Non-initiator methionine tRNA (tRNAMet) was purified from Bacillus subtilis W 168 by a consecutive use of several column chromatographic systems. The nucleotide sequence was determined to be p-G-G-C-G-G-U-G-U-A-G-C-U-C-A-G-C-G-G-C-D-A-G-A-G-C-G-U-A-C-G-G-U-U-C-A-U-m6A-C-C-C -G-U-G-A-G-G(m7G)-U(D)-C-G-G-G-G-G-T-psi-C-G-A-U-C-C-C-C-U-C-C-G-C-C-G-C-U-A-C- C-A-OH. The nucleosides of G46 and U47 were partially modified to m7G and D, respectively. The nucleotide sequence shows a unique feature that the position adjacent to 3'-end of the anticodon C-A-U is occupied by m6A, not by t6A, although the tRNAMet belongs to a groups of tRNAs which recognize codons starting with A.
非起始甲硫氨酸转运核糖核酸（tRNAMet）通过连续使用多种柱色谱系统从枯草芽孢杆菌W168中纯化得到。其核苷酸序列确定为p-G-G-C-G-G-U-G-U-A-G-C-U-C-A-G-C-G-G-C-D-A-G-A-G-C-G-U-A-C-G-G-U-U-C-A-U-m6A-C-C-C -G-U-G-A-G-G(m7G)-U(D)-C-G-G-G-G-G-T-psi-C-G-A-U-C-C-C-C-U-C-C-G-C-C-G-C-U-A-C- C-A-OH。G46和U47的核苷分别部分修饰为m7G和D。该核苷酸序列显示出一个独特的特征，尽管tRNAMet属于识别以A开头密码子的tRNA组，但反密码子C-A-U的3'-末端相邻位置被m6A占据，而非t6A。
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