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嗜热四膜虫中三种核编码谷氨酰胺tRNA中只有一种的线粒体导入。

Mitochondrial import of only one of three nuclear-encoded glutamine tRNAs in Tetrahymena thermophila.

作者信息

Rusconi C P, Cech T R

机构信息

Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309-0215, USA.

出版信息

EMBO J. 1996 Jul 1;15(13):3286-95.

PMID:8670829
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC451891/
Abstract

The mitochondrial genome of Tetrahymena does not appear to encode enough tRNAs to perform mitochondrial protein synthesis. It has therefore been proposed that nuclear-encoded tRNAs are imported into the mitochondria. T.thermophila has three major glutamine tRNAs: tRNA(Gln)(UUG), tRNA(Gln)(UUA) and tRNA(Gln)(CUA). Each of these tRNAs functions in cytosolic translation. However, due to differences between the Tetrahymena nuclear and mitochondrial genetic codes, only tRNA(Gln)(UUG) has the capacity to function in mitochondrial translation as well. Here we show that approximately 10-20% of the cellular complement of tRNA(Gln)(UUG) is present in mitochondrial RNA fractions, compared with 1% or less for the other two glutamine tRNAs. Furthermore, this glutamine tRNA is encoded only by a family of nuclear genes, the sequences of several of which are presented. Finally, when marked versions of tRNA(Gln)(UUG) and tRNA(Gln)(UUA) flanked by identical sequences are expressed in the macronucleus, only the former undergoes mitochondrial import; thus sequences within tRNA(Gln)(UUG) direct import. Because tRNA(Gln)(UUG) is a constituent of mitochondrial RNA fractions and is encoded only by nuclear genes, and because ectopically expressed tRNA(Gln)(UUG) fractionates with mitochondria like its endogenous counterpart, we conclude that it is an imported tRNA in T.thermophila.

摘要

嗜热四膜虫的线粒体基因组似乎并未编码足够数量的tRNA来进行线粒体蛋白质合成。因此，有人提出由细胞核编码的tRNA会被导入线粒体。嗜热栖热菌有三种主要的谷氨酰胺tRNA：tRNA(Gln)(UUG)、tRNA(Gln)(UUA)和tRNA(Gln)(CUA)。这些tRNA中的每一种都在胞质翻译中发挥作用。然而，由于嗜热四膜虫细胞核和线粒体遗传密码之间存在差异，只有tRNA(Gln)(UUG)也有能力在线粒体翻译中发挥作用。在此我们表明，tRNA(Gln)(UUG)的细胞总量中约有10% - 20%存在于线粒体RNA组分中，而其他两种谷氨酰胺tRNA的这一比例为1%或更低。此外，这种谷氨酰胺tRNA仅由一个核基因家族编码，文中给出了其中几个基因的序列。最后，当侧翼带有相同序列的tRNA(Gln)(UUG)和tRNA(Gln)(UUA)的标记版本在大核中表达时，只有前者会发生线粒体导入；因此tRNA(Gln)(UUG)中的序列指导导入过程。由于tRNA(Gln)(UUG)是线粒体RNA组分的一个组成部分，且仅由核基因编码，并且由于异位表达的tRNA(Gln)(UUG)与其内源性对应物一样与线粒体共分离，我们得出结论，它是嗜热栖热菌中一种被导入的tRNA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e00/451891/0eab31c762c1/emboj00013-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e00/451891/46efbc5d3dc2/emboj00013-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e00/451891/bad2cf96390d/emboj00013-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e00/451891/43ae242d434b/emboj00013-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e00/451891/0eab31c762c1/emboj00013-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e00/451891/46efbc5d3dc2/emboj00013-0082-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e00/451891/bad2cf96390d/emboj00013-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e00/451891/43ae242d434b/emboj00013-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e00/451891/0eab31c762c1/emboj00013-0085-a.jpg

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