O'Donohue T L, Handelmann G E, Chaconas T, Miller R L, Jacobowitz D M
Peptides. 1981 Fall;2(3):333-44. doi: 10.1016/s0196-9781(81)80126-x.
alpha-MSH immunoreactive peptides were fractionated and characterized in rat and human brain and rat pituitary by reversed phase high pressure liquid chromatographic techniques. alpha-MSH and deacetylated alpha-MSH were two major naturally existing peptides in both brain and pituitary gland. Subsequent experiments examined the roles of these two peptides in neuronal function. The alpha-MSH was clearly more effective than deacetylated alpha-MSH in improving performance on a visual discrimination task after intraperitoneal administration and in inducing excessive grooming after intraventricular administration. The difference in behavioral potency may be explained by the fact that alpha-MSH was much more resistant to peptidase degradation than was deacetylated alpha-MSH. N-acetylation of alpha-MSH may be an effective regulatory process for modulating the behavioral potency of the secretory product of alpha-MSH-containing pituitary cells and neurons.
通过反相高压液相色谱技术对大鼠和人类大脑以及大鼠垂体中的α-促黑素免疫反应性肽进行了分离和表征。α-促黑素和脱乙酰化α-促黑素是大脑和垂体中两种主要的天然存在的肽。随后的实验研究了这两种肽在神经元功能中的作用。腹腔注射后，α-促黑素在改善视觉辨别任务表现方面明显比脱乙酰化α-促黑素更有效；脑室内注射后，α-促黑素在诱导过度梳理行为方面也更有效。行为效力的差异可能是由于α-促黑素比脱乙酰化α-促黑素对肽酶降解的抵抗力更强。α-促黑素的N-乙酰化可能是调节含α-促黑素的垂体细胞和神经元分泌产物行为效力的有效调节过程。
