Hemminki K
Toxicol Lett. 1981 Oct;9(2):161-4. doi: 10.1016/0378-4274(81)90034-5.
Formaldehyde reacted with guanosine and the products were assayed using fluorescence spectroscopy at different pHs. The reaction products were fluorescent in alkali only as was found with commercial N-2 methylguanosine. Thus, formaldehyde appeared to react with N-2 of guanosine. The reaction was completed with 30s both at 0 degrees C or at 21 degrees C. The stability of the reaction products was further investigated by the addition of glycine. When formaldehyde was allowed to react with guanosine for 30s, 90% of the fluorescence disappeared when glycine was added suggesting a labile adduct such as a methylol derivative. By contrast, after a 20 h incubation only 20% of fluorescence was abolished with glycine suggesting a stable adduct such as a methylene derivative.
甲醛与鸟苷反应，产物在不同pH值下用荧光光谱法进行检测。反应产物仅在碱性条件下有荧光，这与市售的N - 2 - 甲基鸟苷情况相同。因此，甲醛似乎与鸟苷的N - 2位发生了反应。该反应在0℃或21℃下30秒内完成。通过添加甘氨酸进一步研究了反应产物的稳定性。当甲醛与鸟苷反应30秒后，加入甘氨酸时90%的荧光消失，这表明形成了不稳定的加合物，如羟甲基衍生物。相比之下，孵育20小时后，加入甘氨酸仅使20%的荧光消失，这表明形成了稳定的加合物，如亚甲基衍生物。
