霍乱肠毒素的分子特性

Molecular properties of cholera enteroxin.

作者信息

Okajima Y, Shimamura T, Ozawa A, Sasaki S

出版信息

Tokai J Exp Clin Med. 1981 Apr;6(2):193-201.

Abstract

The molecular properties of Vibrio cholerae enterotoxin obtained from two different insitutions were studied. The preparations, which were purified by different methods, showed the same physicochemical properties. Ultracentrifugal analysis showed that the toxin had a molecular weight of 79,000, a sedimentation coefficient of 5.25 S and a diffusion coefficient of 6.02 D. When the toxin was subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, the A subunit with an apparent molecular weight of 31,000-32,000 was observed. Based of these findings and the molecular weight of the B subunit of 11,604 which was calculated from its primary structure, it is proposed that holotoxin is composed of one A subunit and four B subunits.

摘要

对从两个不同机构获得的霍乱弧菌肠毒素的分子特性进行了研究。通过不同方法纯化的制剂显示出相同的物理化学性质。超速离心分析表明，该毒素的分子量为79,000，沉降系数为5.25 S，扩散系数为6.02 D。当毒素在十二烷基硫酸钠存在下进行聚丙烯酰胺凝胶电泳时，观察到表观分子量为31,000 - 32,000的A亚基。基于这些发现以及根据其一级结构计算出的B亚基分子量11,604，推测全毒素由一个A亚基和四个B亚基组成。