Grossman H B, Kleinert E L, Lesser M L, Herr H W, Whitmore W F
Urol Res. 1981;9(5):237-40. doi: 10.1007/BF00256893.
This study was undertaken to determine whether hormonal stimulation followed by chemotherapy with a cell-cycle specific agent would improve the effectiveness of the chemotherapy in a prostatic adenocarcinoma model. One hundred Copenhagen rats were randomised into 5 equal groups and injected subcutaneously with 2 x 10(7) cells of Dunning G strain prostatic adenocarcinoma. The groups were treated in the following fashion: 1. sham operated controls, 2. castration, 3. castration and methotrexate, 4. castration, testosterone and methotrexate and 5. castration and testosterone. When the tumours became palpable, all animals received the surgery to which they were randomised. Subsequent hormonal and chemotherapy was started 1 week thereafter. Therapy was given for 5 consecutive days followed by a 16-day recovery period and then continued in a cyclical fashion. Serial measurements of animal weights and tumour size were obtained. Analysis of tumour growth was restricted to the first 29 days of therapy because of a rapid decline in animal survival beyond that point. The group treated with castration, testosterone, and methotrexate inhibited tumour growth more than any other group and was the only group that was significantly different from control (P less than 0.05).
本研究旨在确定在前列腺腺癌模型中，激素刺激后使用细胞周期特异性药物进行化疗是否会提高化疗效果。将100只哥本哈根大鼠随机分为5组，每组数量相等，皮下注射2×10⁷个邓宁G株前列腺腺癌细胞。分组及处理方式如下：1. 假手术对照组；2. 去势组；3. 去势加甲氨蝶呤组；4. 去势、睾酮加甲氨蝶呤组；5. 去势加睾酮组。当肿瘤可触及后，所有动物接受其随机分组对应的手术。术后1周开始后续的激素及化疗。连续给药5天，随后有16天的恢复期，然后以周期方式继续给药。对动物体重和肿瘤大小进行连续测量。由于该时间点之后动物存活率迅速下降，因此肿瘤生长分析仅限于治疗的前29天。去势、睾酮加甲氨蝶呤组抑制肿瘤生长的效果比其他任何组都好，并且是唯一与对照组有显著差异的组（P<0.05）。
