Wadeleux P A, Winand R J
Acta Endocrinol (Copenh). 1981 Dec;98(4):533-9. doi: 10.1530/acta.0.0980533.
In cultured cells of normal dog thyroid thyrotrophin (TSH) has been shown to have a mitogenic effect. The present study suggests an opposite action in cells from human non-toxic goitre. After incubation of these cells with [3H]thymidine, a decreased incorporation into DNA is observed in the presence of TSH. Whereas in dog thyroid cells the hormone enhances the incorporation of [3H]leucine and [14C]glucosamine into macromolecules, TSH inhibits the incorporation by human non-toxic goitre cells. Polyacrylamide gel electrophoresis analysis of the proteins present in the cell layer or released in the culture medium shows that the inhibition affects all the newly synthesized peptides, including the radioactive material co-migrating with the major band (330 000 daltons) observed with human purified thyroglobulin. While several hydrolase activities are not influenced by TSH in dog thyroid cells, the hormone markedly decreases the activities of some hydrolases of thyroid cells from human non-toxic goitre. Our results therefore suggest that, in cultured thyroid cells from human non-toxic goitre, TSH induces a resting state of the cells, characterized by reduced growth, reduced protein synthesis and reduced thyroglobulin hydrolysis.
在正常犬甲状腺的培养细胞中，促甲状腺激素（TSH）已被证明具有促有丝分裂作用。本研究表明，在来自人类非毒性甲状腺肿的细胞中，TSH具有相反的作用。在用[3H]胸腺嘧啶核苷孵育这些细胞后，在TSH存在的情况下，观察到DNA掺入量减少。在犬甲状腺细胞中，该激素可增强[3H]亮氨酸和[14C]葡糖胺掺入大分子的过程，而TSH则抑制人类非毒性甲状腺肿细胞的掺入。对细胞层中或培养基中释放的蛋白质进行聚丙烯酰胺凝胶电泳分析表明，这种抑制作用影响所有新合成的肽，包括与人类纯化甲状腺球蛋白中观察到的主要条带（330 000道尔顿）共迁移的放射性物质。虽然在犬甲状腺细胞中几种水解酶活性不受TSH影响，但该激素可显著降低人类非毒性甲状腺肿甲状腺细胞中某些水解酶的活性。因此，我们的结果表明，在来自人类非毒性甲状腺肿的培养甲状腺细胞中，TSH诱导细胞进入静止状态，其特征为生长减少、蛋白质合成减少和甲状腺球蛋白水解减少。
