Rousset B, Munari Y, Mornex R
Ann Endocrinol (Paris). 1975 May-Jun;36(3):163-4.
We present here data on hormone synthesis and thyroxine secretion by isolated human thyroid cells. Thyroid cells were dispersed by trypsinization. Thyroid tissue was surgically obtained from two euthyroid patients with hyperplastic goitre and from two thyrotoxic patients with toxic adenoma. In 6 hr incubation experiments, we observed that isolated human thyroid cells, i) concentrated iodide, ii) synthetized iodothyronines, iii) released thyroxine in the incubation medium. Each parameters was TSH dependant. That thyroxine release was due to a secretion process is suggested by similar and further documented data obtained with isolated hog thyroid cells. This technique may provide further help for thyroid physiopathology investigation in humans.
我们在此展示关于分离的人甲状腺细胞激素合成及甲状腺素分泌的数据。甲状腺细胞通过胰蛋白酶消化进行分散。甲状腺组织通过手术取自两名患有增生性甲状腺肿的甲状腺功能正常患者以及两名患有毒性腺瘤的甲状腺毒症患者。在6小时的孵育实验中，我们观察到分离的人甲状腺细胞：i）浓集碘化物；ii）合成碘甲状腺原氨酸；iii）在孵育培养基中释放甲状腺素。每个参数都依赖于促甲状腺激素（TSH）。用分离的猪甲状腺细胞获得的相似且有进一步记录的数据表明，甲状腺素的释放是由于分泌过程。该技术可能为人类甲状腺生理病理学研究提供进一步的帮助。
